Research Statement

In eukaryotes, the 'multivesicular body' (MVB) pathway delivers transmembrane proteins into the lumen of the lysosome for degradation. This degradation pathway is essential for regulating cell surface protein composition, nutrient uptake, cell communication and immune response. Disruption of the MVB sorting pathway can cause significant problems for the cell in responding to extracellular cues and results in pathogenic states such as uncontrolled cellular proliferation in cancers and neurodegenerative diseases. Several multiprotein complexes, called the ESCRTs, execute the sorting of proteins into the MVB pathway. Using yeast as a model system my laboratory combines genetics, cell biology and biochemistry to gain insight into the function of the ESCRT proteins.

Research Keywords

  • Multivesicular body
  • Membrane protein trafficking
  • ESCRTs

Presentations

  • Yale University. Contributed Talk, Presented, 05/2014.
  • ETH Zurich Switzerland. Contributed Talk, Presented, 06/2012.
  • Yale University. Contributed Talk, Presented, 05/2012.
  • University of Osnabrueck Germany. Contributed Talk, Presented, 03/2012.
  • University of Bochum Germany. Contributed Talk, Presented, 03/2012.
  • University of Goettingen Germany. Contributed Talk, Presented, 02/2012.
  • The Children's Hospital of Philadelphia. Contributed Talk, Presented, 02/2011.
  • University of Pennsylvania. Contributed Talk, Presented, 11/2010.
  • Lysosome Gordon Conference, Conference: Biochemistry and Cell Biology of ESCRTs in Health and Disease. Contributed Talk, Presented, 09/2010.
  • University of Goettingen Germany. Contributed Talk, Presented, 09/2010.
  • University of Osnabrueck Germany. Contributed Talk, Presented, 09/2010.
  • Lysosome Gordon Conference, Conference: Biochemistry and Cell Biology of ESCRTs in Health and Disease, University of Goettingen Germany, University of Osnabrueck Germany, University of Pennsylvania. Contributed Talk, Presented, 06/2010.

Research Equipment and Testing Expertise

  • Fluorescence microscope.

Languages

  • German, fluent.

Geographical Regions of Interest

  • Europe

Publications

  • Kim HJ, Jeong MY, Parnell TJ, Babst M, Phillips JD & Winge DR (2016). The Plasma Membrane Protein Nce102 Implicated in Eisosome Formation Rescues a Heme Defect in Mitochondria. The Journal of biological chemistry. Vol. 291, 17417-26. Published, 08/01/2016.
  • Mageswaran SK, Johnson NK, Odorizzi G & Babst M (2015). Constitutively active ESCRT-II suppresses the MVB-sorting phenotype of ESCRT-0 and ESCRT-I mutants. Molecular biology of the cell. Vol. 26, 554-68. Published, 01/01/2015.
  • Babst, M. 2014. Quality control at the plasma membrane: one mechanism does not fit all. J. Cell Biol. 205(1): 11-20. (PMCID24733583). Published, 04/14/2014.
  • Mageswaran, S.K., M.G. Dixon, M. Curtiss, J.P. Keener, and M. Babst. 2014. Binding to any ESCRT can mediate ubiquitin-independent cargo sorting. Traffic 15(2): 212-229. (PMCID24148098). Published, 02/01/2014.
  • Mageswaran, S.K., M.G. Dixon, M. Curtiss, J.P. Keener, and M. Babst. 2013. Binding to any ESCRT can mediate ubiquitin-independent cargo sorting. Traffic, in press. In press, 11/2013.
  • Babst, M., and G. Odorizzi. 2013. The balance of protein expression and degradation: an ESCRTs point of view. Curr. Opin. Cell Biol. 25(4): 489-494. Published, 08/2013.
  • Shestakova, A., M. Curtiss, B.A. Davies, D.J. Katzmann, and M. Babst. 2013. The linker region plays a regulatory role in the assembly and activity of the Vps4 AAA ATPase. J. Biol. Chem. 288(37):26810-26819. Published, 08/2013.
  • Keener, J.M., and M. Babst 2013. Quality control and substrate-dependent downregulation of the nutrient transporter Fur4. Traffic 14(4): 412-427. Published, 02/2013.
  • Jones, C.B., E.M. Ott, J.M. Keener, M. Curtiss, V. Sandrin, and M. Babst. 2012. Regulation of protein degradation by starvation-response pathways. Traffic 13:468-482. Published, 03/2012.
  • Hill, C.P., and M. Babst. 2012. Structure and function of the membrane deformation AAA ATPase Vps4. Biochim. Biophys. Acta. 1823(1):172-181. Published, 02/2012.
  • Babst, M., B.A. Davies, and D.J. Katzmann. 2011. Regulation of Vps4 during MVB sorting and cytokinesis. Traffic 12(10):1298-1305. Published, 02/2011.
  • Babst, M. 2011. MVB vesicle formation: ESCRT-dependent, ESCRT-independent and everything in between. Curr. Opin. Cell Biol. 23(4):452-457. Published, 02/2011.
  • Davies, B.A., I.F. Azmi, J. Payne, A. Shestakova, B.F. Horazdovsky, M. Babst, and D.J. Katzmann. Coordination of substrate binding and ATP hydrolysis in Vps4-mediated ESCRT-III dissociation. Mol. Biol. Cell 21(19):3396-3408. Published, 07/2010.
  • Shestakova, A., A. Hanono, S. Drosner, M. Curtiss, B.A. Davies, D.J. Katzmann, and M. Babst. 2010. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol. Biol. Cell 21(6):1059-71. Published, 01/2010.