CAROL LIM portrait
  • Member, Huntsman Cancer Institute
  • Biological Chemistry Faculty, Pharmaceutical Chemistry
  • Adjunct Professor, Pharmacology And Toxicology
  • Professor, Pharmaceutical Chemistry

Research Keywords

  • Peptide therapy
  • Ovarian Cancer
  • Gene Therapy
  • Chronic myeloid leukemia
  • Breast Cancer
  • Transcription Factors
  • Transcription Factor Based Therapeutics
  • Steroid Hormone Receptors
  • Nuclear Import and Export
  • Gene Expression Regulation
  • DNA/Gene Targeted Therapeutics
  • Cellular Kinetics

Presentations

  • The L.S. Skaggs Biomedical Research Symposium, University of Colorado, Aurora, CO. A Biomolecular Multi-Hit Strategy Targeted Against Bcr-Abl. Invited Talk/Keynote, Presented, 09/28/2012.
  • Departmental Lab Chat: Effectively Using Your Committee (C. Lim and W. Sanders. Panel, Presented, 06/19/2012.
  • University of Maryland, Baltimore, "Targeting Nodal Proteins for Cancer Therapy" . Invited Talk/Keynote, Presented, 01/12/2011.
  • Departmental Lab Chat: Scientific Writing (D. Woessner, C. Lim, and D. Grainger). Panel, Presented, 09/2010.
  • University of Kansas (Lawrence), “Location Alters Function: Making the Switch to Apoptosis” . Invited Talk/Keynote, Presented, 04/2010.
  • Departmental Seminar: Teaching Excellence Seminar (M. Mossalam, C. Lim, and D. Zigenfuss). Panel, Presented, 04/2010.
  • 14th International Symposium on Recent Advances in Drug Delivery Systems, “Controlling Subcellular Localization to Alter Function: Use of the ‘Protein Switch‘ for Cancer Therapy,” Salt Lake City, UT, Feb. 15-18, 2009. Invited Talk/Keynote, Other, 2009.
  • Controlled Release Society 34th Annual Meeting, “Controlled Cellular Delivery of Bcr-Abl Coiled-Coil Domain for Apoptosis of Chronic Myelogenous Leukemia Cells,” Long Beach, CA, July 7-11, 2007 . Invited Talk/Keynote, Other, 2007.

Grants, Contracts & Research Gifts

  • SIMULTANEOUS TARGETING OF P53. PI: LIM,CAROL. NIH NATIONAL CANCER INSTITUTE, 07/01/2010 - 05/31/2014. Total project budget to date: $914,267.00
  • MANIPULATING SIGNAL SEQUENCES. PI: LIM,CAROL. NIH NATIONAL CANCER INSTITUTE, 03/01/2008 - 01/31/2014. Total project budget to date: $1,400,678.00

Languages

  • English, fluent.

Publications

  • Controlled Access of p53 to the Nucleus Regulates Its Proteasomal Degradation by MDM2. Molecular Pharmaceutics, 2013 Mar 1. [Epub ahead of print] PMID:23398638, PMCID in progress. Published, 03/01/2013.
  • A Single Mutant, A276S of p53, Turns the Switch to Apoptosis. Molecular Pharmaceutics, 2013 Mar 1. [Epub ahead of print] PMID: 23402381, PMCID in progress. Published, 03/01/2013.
  • Disrupting BCR-ABL in Combination with Secondary Leukemia-Specific Pathways in CML Cells Leads to Enhanced Apoptosis and Decreased Proliferation, Molecular Pharmaceutics, 2013 Jan 7, 10 (1): 270–277 PMID:2321103; PMCID: PMC3604893 [Available on 2014/1/7]. Published, 01/07/2013.
  • Utilizing the Estrogen Receptor Ligand-Binding Domain for Controlled Protein Translocation to the Insoluble Fraction, Pharmaceutical Research, 2012, Dec;29(12):3455-63; PMID: 22869106; PMCID in progress. Published, 12/2012.
  • Enhanced and Selective Killing of Chronic Myelogenous Leukemia Cells with an Engineered Bcr-Abl Binding Protein and Imatinib, Molecular Pharmaceutics, 2012, Nov 5;9(11):3318-29; PMID:22957899; PMCID: PMC3529210. Published, 11/05/2012.
  • Targeting Malignant Mitochondria with Therapeutic Peptides. Therapeutic Delivery, 2012 Aug, 3(8): 861-979. DOI 10.4155/tde.12.75, PMID:22946430. Published, 08/2012.
  • Selective Targeting of c-Abl via a Cryptic Mitochondrial Targeting Signal Activated by Cellular Redox Status in Leukemic and Breast Cancer Cells, Pharmaceutical Research, 2012 Aug; 29(8):2317-28 [Epub 2012 May 2]. PMID: 22549737; PMCID: PMC3399985. Published, 08/2012.
  • Changing the Subcellular Location of the oncoprotein Bcr-Abl Using Rationally Designed Capture Motifs, Pharmaceutical Research, 2012, 29(4):1098-1109. PMID 22183511. Published, 04/2012.
  • Changing the Subcellular Location of the oncoprotein Bcr-Abl Using Rationally Designed Capture Motifs, Pharmaceutical Research, 2012, 29(4):1098-1109. PMID 22183511. Published, 04/2012.
  • Direct Induction of Apoptosis Using an Optimally Mitochondrially Targeted p53, Molecular Pharmaceutics, 2012 March 1 [Epub ahead of print]. PMID 22380534. Published, 03/01/2012.
  • Cancer Biology: Some Causes for a Variety of Different Diseases, invited book chapter in "Cancer Targeted Drug Delivery: Elusive Dream," editors Bae, Y.H., Mrsny, R., and Park, K. (Springer), in press. In press, 01/2012.
  • Improved Coiled-Coil Design Enhances Interaction with Bcr-Abl and Induces Apoptosis, Molecular Pharmaceutics, 2012 Jan 1, 9(1):187-195. PMID 22136227. Published, 01/01/2012.
  • Development of an Effective Therapy for CML, The Cancer Journal: The Journal of Principles & Practice of Oncology. Theme issue "Frontiers of Personalized Medicine," 17(6):477-486. PMID: 22157291. Published, 11/2011.
  • The Androgen Receptor and Its Use in Biological Assays: Looking Toward Effect-Based Testing and Its Applications. The Journal of Analytical Toxicology, 2011, 35(9): 594-607. PMID: 22080898. Published, 11/2011.
  • Disruption of Bcr-Abl Coiled-Coil Oligomerization By Design, Journal of Biological Chemistry, 2011 Aug 5;286(31):27751-60. PMID: 21659527. Published, 08/2011.
  • "Choosing Targets for Gene Therapy" in the book "Targets for Gene Therapy," published by Intech Open Access Publisher, July 2011, ISBN 978-953-307-540-2. Published, 07/2011.
  • The Nuclear Translocation Assay for Intracellular Protein-Protein Interactions and its Application to the Bcr Coiled-Coil Domain. Biotechniques, 2010 Jul;49(1):519-24. Published, 07/2010.
  • Mossalam, M., Dixon, A.S., and Lim, C.S. Controlling Subcellular Delivery to Optimize Therapeutic Effect, Therapeutic Delivery, July 2010, 1(1):169-193. Published, 07/2010.
  • Effect of Anabolic-Androgenic Steroids and Glucocorticoids on the Kinetics of hAR and hGR Nucleocytoplasmic Translocation. Molecular Pharmaceutics, 2010 Mar 15. [Epub ahead of print] PMID: 20230007. In press, 03/15/2010.
  • Dixon, A.S., Kakar, M, Schneider, K.M.H., Constance, J.E., Paullin, B.C., and Lim, C.S. Controlling Subcellular Localization to Alter Function: Sending Oncogenic Bcr-Abl to the Nucleus Causes Apoptosis, Journal of Controlled Release, 2009. 140(3): 245-9. Published, 2009.
  • Kakar, M., Davis, J.R., Kern, S.E., and Lim, C.S. Optimizing the protein switch: altering nuclear import and export signals, and ligand binding domain, Journal of Controlled Release, 2007 Jul 31;120(3):220-32. Epub 2007 May 3. Published, 2007.
  • Lim, C.S. Organelle-specific targeting in drug delivery and design. Advanced Drug Delivery Reviews, 2007, 59(8):697. Theme editor also of this issue. Published, 2007.
  • Kakar, M., Cadwallader, A.B., Davis, J.R., and Lim, C.S. Signal sequences for targeting of gene therapy products to subcellular compartments: The role of CRM1 in nucleocytoplasmic shuttling of the protein switch. Pharm Res, 2007 Jun 13; [Epub ahead of print]. Published, 2007.
  • Kakar, M., Kanwal, C., Davis, J.R., Li, H. and Lim, C. S. Geldanamycin, an inhibitor of hsp90, blocks cytoplasmic retention of progesterone receptor and glucocorticoid receptor via their respective ligand binding domains, AAPS Journal, 2006;8(4): E718-E728.DOI: 10.1208/aapsj080481. Published, 2006.
  • Davis, J.R., Kakar, M., and Lim, C.S. Controlling Protein Compartmentalization to Overcome Disease. Pharmaceutical Research, 24(1) January, 2007, Epub Sep 13 2006. Published, 2006.
  • Li, H., Fidler, M. L., and Lim, C. S. Effect of Initial Subcellular Localization of Progesterone Receptor on Import Kinetics and Transcriptional Activity, Molecular Pharmaceutics, 2005, Nov-Dec;2(6):509-18. Published, 2005.
  • Kanwal, C., Mu, S. and Lim, C.S. Bidirectional On/Off Switch for Controlled Targeting of Proteins to Subcellular Compartments, Journal of Controlled Release, 98(3):379-393, 2004. Published, 2004.
  • Li H., Yan G., Kern S.E. and Lim C.S., Correlation Among Agonist Dose, Rate of Import, and Transcriptional Activity of Liganded Progesterone Receptor B Isoform in Living Cells, Pharmaceutical Research, 20(10):1574-1580, 2003. Published, 2003.
  • Kanwal C., Li H. and Lim C.S., Model System to Study Classical Nuclear Export Signals, AAPS PharmSci 2002; 4 (3) article 18, 1-8. (http://www.aapspharmsci.org/scientificjournals/pharmsci/journal/ps040318.htm). Published, 2002.
  • Baumann, C.T., Ma, H., Wolford, R., Reyes, J., Maruvda, P., Lim, C., Yen, P.M., Stallcup, M.R., and Hager, G.L. The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome. Molecular Endocrinology, 15(4):485-400, 2001. Published, 2001.
  • Hager, G.L., Lim, C.S., Elbi, C., and Baumann, C.T. Trafficking of Nuclear Receptors in Living Cells. Journal of Steroid Biochemistry and Molecular Biology, 74(5), 249-254, 2000. Published, 2000.
  • Lim, C.S., Baumann, C.T., Htun, H., Xian, W., Irie, M., Smith, C.L., and Hager, G.L. Differential localization of the A and B forms of the human progesterone receptor, Molecular Endocrinology, 13(3): 366-375, 1999. Published, 1999.
  • Baumann, C.T., Lim, C.S., and Hager, G.L. Intracellular localization and trafficking of steroid hormone receptors, Cell Biochemistry and Biophysics, 31(2):119-27, 1999. Published, 1999.
  • Lim, C.S., Baumann, C.T. (co-first authors), and Hager, G.L. Simultaneous visualization of the yellow and green forms of GFP in living cells. Journal of Histochemistry and Cytochemistry, 46(9): 1073-1076, 1998. Published, 1998.
  • Lim, C.S., Jabrane-Ferrat, N., Fontes, J.D., Okamoto, H., Garovoy, M.R., Peterlin, B.M., and Hunt, C.A. Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys. Nucleic Acids Research, 25 (3): 575-581, 1997. Published, 1997.
  • Lim, C.S. and Hunt, C.A. Synthesis of DNA dumbbells: chemical vs. enzymatic ligation of self-complementary oligonucleotides. Nucleosides and Nucleotides, 16 (1-2): 41-51, 1997. Published, 1997.
  • Guy, R.H., Kalia, Y.N., Lim, C.S., Nonato, L.B., and Turner, N.G. Drug smuggling- creative ways to cross biological barriers. Chemistry in Britain, 32(7): 42-45, 1996. Published, 1996.
  • Lim, C.S. and Hunt, C.A. Sequential staining of short oligonucleotides in polyacrylamide mini-gels: using ethidium bromide and methylene blue. Biotechniques, 17(4): 626-628, 1994. Published, 1994.