Publications

  • Sieburth, L.E. (date unknown). Beyond transcription factors: roles of mRNA decay in regulating gene expression in plants. F100Res. Accepted, .
  • (date unknown). Lee, D.L., and Sieburth, L.E. 2010. Plasmodesmata Formation: poking holes in walls with ise. Curr. Biol. In press. Accepted, .
  • (date unknown). Zhang, W., Murphy, C., Sieburth, L. E. 2010. Conserved RNaseII domain protein functions in cytoplasmic mRNA decay and suppresses Arabidopsis decapping mutant phenotypes. Proc. Natl. Acad. Sci. USA. 107: 1598. Accepted, .
  • Lee, DK (date unknown). Lee, Dong-Keun, Van Norman, J. M, Murphy, C., Adhikari, E., Reed, J. W., Sieburth, L. E. 2012. In the absence of BYPASS1-related gene function, the bps signal disrupts embryogenesis by an auxin-independent mechanism. Development 139: 805-815. Accepted, .
  • Roux, ME (date unknown). Roux, M.E., Rasmussen, M.W., , Palma, K., Lolle, S., Regué, AM., Bethke, G., Glazebrook, J., Zhang, W., Sieburth, L, Larsen, M.R., Mundy, J., and Petersen, M. 2015. The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2. EMBO J. 34: 593-608. Accepted, .
  • Adhikari, E. (date unknown). Adhikari, E., Lee, D.-K., Giavalisco, P., and Sieburth, L.E. 2013. Long-distance signaling in bypass1 mutants: bioassay development reveals the bps signal to be a metabolite. Molecular Plant. 6 (1): 164-173. Accepted, .
  • Lee, DK (date unknown). Lee, D-K, and Sieburth, L.E. 2012. Article Addendum: The bps signal: Embryonic arrest from an auxin-independent mechanism in bypass triple mutants. Plant Signaling & Behavior. 7:698-700. Accepted, .
  • Lee, D.H. (date unknown). Lee, D.H., Parrott, D.L., Adhikari, E., Fraser, N., and Sieburth, L.E. The mobile bypass Signal Arrests Shoot Growth by disrupting SAM Maintenance, Cytokinin Signaling, and WUS Expression. 2016. Plant Physiology, 171: 2178-2190. Accepted, .
  • Thanin Chantarachot (date unknown). DHH1/DDX6-like RNA helicases maintain ephemeral half-lives of stress-response mRNAs associated with innate immunity and growth inhibition. Nature Plants. Accepted, .
  • (date unknown). Van Norman, J.M., Murphy, C. Sieburth, L.E. 2011. BYPASS1: synthesis of the mobile root-derived signal requires active root growth and arrests early leaf development. BMC Plant Biol. 11:28. Accepted, .
  • Carlos Perea-Resa (date unknown). Carlos Perea-Resa, Cristian Carrasco-López1, Rafael Catalá, Veronika Turečková, Ondrej Novak, Weiping Zhang, Leslie Sieburth, José Manuel Jiménez-Gómez and Julio Salinas. 2016. The Lsm1-7 Complex Controls Plant Adaptation To Adverse Environmental Conditions By Promoting Selective mRNA Decapping. Plant Cell. 28: 505 – 520. Accepted, .
  • Sorenson, R.S (date unknown). Arabidopsis mRNA decay landscape arises from specialized RNA decay substrates, decapping-mediated feedback, and redundancy. Proceedings of National Academy USA. Accepted, .
  • (date unknown). Sieburth, L.E., and Lee, D.K. 2010. BYPASS1: How a Tiny Mutant Tells a Big Story about Root-to-shoot Signaling. J. Integrative Plant Biol. 52:77-85. Accepted, .

Research Statement

We use Arabidopsis as our model system, and a decade ago we carried out screens to identify mutants with defects in patterning of the leaf vascular system.  This screen yielded a rich assortment of mutants, and resulted in two major funded projects in my lab.  One project centers around the bypass1 (bps1) mutant, whose characterization led us to identify a root-to-shoot signaling pathway that appears to involve a novel signaling molecule with properties resembling a plant hormone.  We are currently working to identify the signaling molecule, its role in shoot development, and to characterize the cell biology of BPS1 proteins.   The other project arose from characterization of varicose (vcs) and trident (tdt) mutants, which have defects in mRNA decapping components.  Decapping removes the protective 7-methyl guanine nucleotide attached by a 5’-5’ triphosphate linkage (the cap) from mRNAs, which initiates 5’-to-3’ decay.  Traditionally the major model for mRNA decay, and especially decapping, has been yeast (Saccharomyces cerevisiae).  However, the decapping complex of both plants and animals contain a large scaffold (VCS) that is missing from yeast, and this plant/animal complex also carries out additional miRNA-related functions.   Our contributions to this field include the first demonstration that general cytoplasmic mRNA decay pathways have substrate specificity, elucidating roles for the decapping complex in miRNA directed translational regulation,  and identification of a novel cytoplasmic mRNA decay pathway that is conserved in plants and animals (but again missing in yeast).
 

Research Keywords

  • Systematic Biology
  • Water Pollution
  • Environmental Restoration or Remediation
  • Automata
  • Watersheds

Presentations

  • Bioimaging Science Program Meeting. Invited Speaker. Novel in-vivo visualization of bioenergy metabolic and cellular phenotypes in living woody tissue. , Presented, 2022.
  • Wake Forest University, Department of Biochemistry. The roles of mRNA decay in shaping transcriptome responses to a stimulus. , Presented, 2021.
  • Plant Canada 2019, Guelph, Ontario. Keynote address. Beyond transcription factors: a degrading story of gene expression control. , Presented, 2019.
  • Seminar to the Cell and Molecular Biology division: Tales from the crypt: what we can learn from [mRNA] decay. , Presented, 2018.
  • Seminar to the Plant Biology division. The bps signal communicates drought perception from the root. , Presented, 2018.
  • KSAB International Conference on Plant Molecular Biology, PyongChang, South Korea. , Presented, 2015.
  • University of Florida, Institute of Genetics. , Presented, 2015.
  • Korean Society for Agricultural Biochemistry, 50th Year Conference. Invited Speaker Long Distance Signaling in Plants. , Presented, 2010.
  • Seminar at Wake Forest University, Department of Biology, Molecular Biology and Biochemistry Group. Title: Gene Expression: the roles of mRNA decay during steady state and during a stimulus response. , Presented, 2020.
  • Seminar, Purdue University, Department of Plant Sciences. BYPASS1: Regulator of a Long-Distance Signaling Pathway with links to Drought signaling. , Presented, 2019.
  • University of Arizona, Tucson. March 2017. BYPASS1: REGULATOR OF A LONG-DISTANCE SIGNALING PATHWAY WITH LINKS TO DROUGHT. , Presented, 2016.
  • Yonsei University Seminar Long-Distance Signaling in Plants: the BPS1 protein is a negative regulator. , Presented, 2010.

Research Groups

  • Jessica Vincent, . 2017 - present.
  • Alex Cummins, . School of Biological Sciences. 2016 - present.
  • Reed Sorenson, . School of Biological Sciences. 2016 - present.

Software Titles

  • RNAdecay: Maximum Likelihood Decay Modeling of RNA Degradation Data. R package version 1.2.1. This r package uses RNAseq data to calculate two parameters of mRNA decay: the initial decay rate, and a second factor, beta, which quantifies slowing of decay rate over the course of the experiment. The package includes sample data. Release Date: 2018. Inventors: Sorenson, R., Johnson, K., Adler, F., Sieburth, L.