Profile Picture
  • Adjunct Associate Professor, Internal Medicine
  • Adjunct Associate Professor, Department Of Biochemistry
  • Professor, Nutrition & Integrative Physiology

Research Statement

The Boudina Laboratory we are interested in understanding the molecular mechanisms involved in weight gain regulation and adipose tissue expansion in diet-induced obesity (DIO). Our laboratory is focused on (1) the understanding of the role of reactive oxygen species (ROS) in fat development in DIO in mice, and (2) to study the effect of dietary on white adipose progenitor cells proliferation and differentiation.


  • Forostyan T, Pires K, Buffalo M, Kanishka L, Graham T, Boudina S. (2017). Deletion of the RBP4 receptor STRA6L in CD11c+ immune cells protects mice from diet-induced glucose intolerance and insulin resistance. Poster session presented at Keystone Resort, Keystone, Colorado Discounted Registration Deadline: November 22, 2016, Colorado. Poster, Presented, 01/2017.
  • Ortega S, Buffolo M, Pires KM, and Boudina S. (2017). Mitochondrial Stress: A Novel Thermogenic Pathway to Fight Obesity and Diabetes. Poster session presented at J4: Obesity and Adipose Tissue Biology, Keystone Resort, Keystone, Colorado. Poster, Presented, 01/2017.
  • Pires KM, Ilkun O, Buffolo MA, Pei S, Abel ED, and Boudina S. (2016). Activation of IGF-1 receptor signaling by hyperinsulinemia suppresses cardiac autophagy through TRB3/p62 pathway. Poster, Presented, 11/2016.
  • Pires KM, Ilkun O, Buffolo M, Pei S, and Boudina S. (2016). Hyperinsulinemia signals though the IGF1-ERK pathway to suppress autophagy in the insulin resistant heart. Poster session presented at MITOCHONDRIAL BIOLOGY SYMPOSIUM: NOVEL ROLES OF MITOCHONDRIA IN HEALTH AND DISEASE, NATIONAL INSTITUTE OF HEALTH, BETHESDA, MARYLAND. Poster, Presented, 05/2016.
  • Han YH, Buffolo M, Pires KM, Pei S, and Boudina S. (2016). Mitochondrial Stress: A Novel Pathway to Activate Thermogenesis in Fat. Poster session presented at MITOCHONDRIAL BIOLOGY SYMPOSIUM: NOVEL ROLES OF MITOCHONDRIA IN HEALTH AND DISEASE, NATIONAL INSTITUTE OF HEALTH, BETHESDA, MARYLAND. Poster, Presented, 05/2016.

Grants, Contracts & Research Gifts

  • Novel pathways for fat burning that protects from high fat diet-induced metabolic (R01DK098646). PI: BOUDINA, SIHEM. National Institutes of Health, 2013 - 08/31/2015. Total project budget to date: $648,150.00
  • Novel pathways for fat burning that protects from high fat diet-induced metabolic (R01DK098646). PI: BOUDINA, SIHEM. National Institutes of Health, 2013 - 08/31/2015. Total project budget to date: $648,150.00
  • Mechanisms by Which ROS Regulate Adipogenesis and Fat Expansion in Diet-Induced Obesity. PI: Boudina, Sihem PhD. American Heart Association, 2009 - 06/30/2013. Total project budget to date: $308,000.00
  • Mechanisms by Which ROS Regulate Adipogenesis and Fat Expansion in Diet-Induced Obesity. PI: Boudina, Sihem PhD. American Heart Association, 2009 - 06/30/2013. Total project budget to date: $308,000.00


  • English, fluent.


  • Pires KM, Buffolo M, Schaaf C, Symons JD , Cox J, Abel ED, Selzman CH & Boudina S (2017). Activation of IGF-1 receptors and Akt signaling by systemic hyperinsulinemia contributes to cardiac hypertrophy but does not regulate cardiac autophagy in obese diabetic mice. J Mol Cell Cardiol. Vol. 113, 39-50. Published, 08/05/2017.
  • Bharath LP , Cho JM, Park SK, Ruan T, Li Y, Muellar R, Bean T, Reese V, Richardson RS, Cai J, Sargsyan A, Pires K, Anandh Babu PV, Boudina S, Graham TE & Symons JD (2017). Endothelial Cell Autophagy Maintains Shear Stress-Induced Nitric Oxide Generation via Glycolysis-Dependent Purinergic Signaling to Endothelial Nitric Oxide Synthase . Arterioscler Thromb Vasc Biol. Vol. 37(9), 1646-1656. Published, 07/06/2017.
  • Ortega SP, Chouchani ET, Boudina S (2017). Stress Turns on the Heat: Regulation of Mitochondrial Biogenesis and UCP1 by ROS in Adipocytes. Adipocyte. Vol. 6, Issue 1, 56-61. Published, 01/2017.
  • Han YH, Buffolo M, Pires KM, Pei S, Scherer PE & Boudina S (2016). Adipocyte-Specific Deletion of Manganese Superoxide Dismutase Protects From Diet-Induced Obesity Through Increased Mitochondrial Uncoupling and Biogenesis. Diabetes. Vol. 65, 2639-51. Published, 08/01/2016.
  • Park H, Cho S, Han YH, Janat-Amsbury MM, Boudina S & Bae YH (2015). Combinatorial gene construct and non-viral delivery for anti-obesity in diet-induced obese mice. Journal of controlled release : official journal of the Controlled Release Society. Vol. 207, 154-62. Published, 05/01/2015.
  • Singhal AK, Symons JD, Boudina S, Jaishy B & Shiu YT (2015). Role of Endothelial Cells in Myocardial Ischemia-Reperfusion Injury. Vascular disease prevention. Vol. 7, 1-14. Published, 01/01/2015.
  • Boudina S & Graham TE (2014). Mitochondrial function/dysfunction in white adipose tissue. Experimental physiology. Vol. 99, 1168-78. Published, 09/01/2014.
  • Pires KM, Ilkun O, Valente M & Boudina S (2014). Treatment with a SOD mimetic reduces visceral adiposity, adipocyte death, and adipose tissue inflammation in high fat-fed mice. Obesity (Silver Spring, Md.). Vol. 22, 178-87. Published, 01/01/2014.
  • Silva FJ, Holt DJ, Vargas V, Yockman J, Boudina S, Atkinson D, Grainger DW, Revelo MP, Sherman W, Bull DA & Patel AN (2014). Metabolically active human brown adipose tissue derived stem cells. Stem cells (Dayton, Ohio). Vol. 32, 572-81. Published, 01/01/2014.
  • Fullmer TM, Pei S, Zhu Y, Sloan C, Manzanares R, Henrie B, Pires KM, Cox JE, Abel ED & Boudina S (2013). Insulin suppresses ischemic preconditioning-mediated cardioprotection through Akt-dependent mechanisms. Journal of molecular and cellular cardiology. Vol. 64, 20-9. Published, 10/01/2013.
  • Boudina S (2013). Cardiac aging and insulin resistance: could insulin/insulin-like growth factor (IGF) signaling be used as a therapeutic target?. Current pharmaceutical design. Vol. 19, 5684-94. Published, 08/01/2013.
  • Ilkun O & Boudina S (2013). Cardiac dysfunction and oxidative stress in the metabolic syndrome: an update on antioxidant therapies. Current pharmaceutical design. Vol. 19, 4806-17. Published, 07/01/2013.
  • Dodson MV, Boudina S, Albrecht E, Bucci L, Culver MF, Wei S, Bergen WG, Amaral AJ, Moustaid-Moussa N, Poulos S & Hausman GJ (2013). A long journey to effective obesity treatments: is there light at the end of the tunnel?. Experimental biology and medicine (Maywood, N.J.). Vol. 238, 491-501. Published, 07/01/2013.
  • Boudina S, Han YH, Pei S, Tidwell TJ, Henrie B, Tuinei J, Olsen C, Sena S & Abel ED (2012). UCP3 regulates cardiac efficiency and mitochondrial coupling in high fat-fed mice but not in leptin-deficient mice. Diabetes. Vol. 61, 3260-9. Published, 11/01/2012.
  • Ishiwata T, Orosz A, Wang X, Mustafi SB, Pratt GW, Christians ES, Boudina S, Abel ED & Benjamin IJ (2012). HSPB2 is dispensable for the cardiac hypertrophic response but reduces mitochondrial energetics following pressure overload in mice. PloS one. Vol. 7, e42118. Published, 08/01/2012.
  • Bricker DK, Taylor EB, Schell JC, Orsak T, Boutron A, Chen YC, Cox JE, Cardon CM, Van Vranken JG, Dephoure N, Redin C, Boudina S, Gygi SP, Brivet M, Thummel CS & Rutter J (2012). A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans. Science (New York, N.Y.). Vol. 337, 96-100. Published, 07/01/2012.
  • Boudina S, Sena S, Sloan C, Tebbi A, Han YH, O'Neill BT, Cooksey RC, Jones D, Holland WL, McClain DA & Abel ED (2012). Early mitochondrial adaptations in skeletal muscle to diet-induced obesity are strain dependent and determine oxidative stress and energy expenditure but not insulin sensitivity. Endocrinology. Vol. 153, 2677-88. Published, 05/01/2012.
  • Bugger H, Riehle C, Jaishy B, Wende AR, Tuinei J, Chen D, Soto J, Pires KM, Boudina S, Theobald HA, Luptak I, Wayment B, Wang X, Litwin SE, Weimer BC & Abel ED (2012). Genetic loss of insulin receptors worsens cardiac efficiency in diabetes. Journal of molecular and cellular cardiology. Vol. 52, 1019-26. Published, 04/01/2012.
  • Li Y, Wende AR, Nunthakungwan O, Huang Y, Hu E, Jin H, Boudina S, Abel ED & Jalili T (2012). Cytosolic, but not mitochondrial, oxidative stress is a likely contributor to cardiac hypertrophy resulting from cardiac specific GLUT4 deletion in mice. The FEBS journal. Vol. 279, 599-611. Published, 01/01/2012.
  • Boudina S & Abel ED (2010). Diabetic cardiomyopathy, causes and effects. Reviews in endocrine & metabolic disorders. Vol. 11, 31-9. Published, 03/01/2010.
  • Wright JJ, Kim J, Buchanan J, Boudina S, Sena S, Bakirtzi K, Ilkun O, Theobald HA, Cooksey RC, Kandror KV & Abel ED (2009). Mechanisms for increased myocardial fatty acid utilization following short-term high-fat feeding. Cardiovascular research. Vol. 82, 351-60. Published, 04/01/2009.
  • Boudina S, Bugger H, Sena S, O'Neill BT, Zaha VG, Ilkun O, Wright JJ, Mazumder PK, Palfreyman E, Tidwell TJ, Theobald H, Khalimonchuk O, Wayment B, Sheng X, Rodnick KJ, Centini R, Chen D, Litwin SE, Weimer BE & Abel ED (2009). Contribution of impaired myocardial insulin signaling to mitochondrial dysfunction and oxidative stress in the heart. Circulation. Vol. 119, 1272-83. Published, 03/01/2009.
  • Bugger H, Boudina S, Hu XX, Tuinei J, Zaha VG, Theobald HA, Yun UJ, McQueen AP, Wayment B, Litwin SE & Abel ED (2008). Type 1 diabetic akita mouse hearts are insulin sensitive but manifest structurally abnormal mitochondria that remain coupled despite increased uncoupling protein 3. Diabetes. Vol. 57, 2924-32. Published, 10/01/2008.
  • Lehman JJ, Boudina S, Banke NH, Sambandam N, Han X, Young DM, Leone TC, Gross RW, Lewandowski ED, Abel ED & Kelly DP (2008). The transcriptional coactivator PGC-1alpha is essential for maximal and efficient cardiac mitochondrial fatty acid oxidation and lipid homeostasis. American journal of physiology. Heart and circulatory physiology. Vol. 295, H185-96. Published, 07/01/2008.
  • Jouihan HA, Cobine PA, Cooksey RC, Hoagland EA, Boudina S, Abel ED, Winge DR & McClain DA (2008). Iron-mediated inhibition of mitochondrial manganese uptake mediates mitochondrial dysfunction in a mouse model of hemochromatosis. Molecular medicine (Cambridge, Mass.). Vol. 14, 98-108. Published, 03/01/2008.
  • Benjamin IJ, Guo Y, Srinivasan S, Boudina S, Taylor RP, Rajasekaran NS, Gottlieb R, Wawrousek EF, Abel ED & Bolli R (2007). CRYAB and HSPB2 deficiency alters cardiac metabolism and paradoxically confers protection against myocardial ischemia in aging mice. American journal of physiology. Heart and circulatory physiology. Vol. 293, H3201-9. Published, 11/01/2007.
  • O'Neill BT, Kim J, Wende AR, Theobald HA, Tuinei J, Buchanan J, Guo A, Zaha VG, Davis DK, Schell JC, Boudina S, Wayment B, Litwin SE, Shioi T, Izumo S, Birnbaum MJ & Abel ED (2007). A conserved role for phosphatidylinositol 3-kinase but not Akt signaling in mitochondrial adaptations that accompany physiological cardiac hypertrophy. Cell metabolism. Vol. 6, 294-306. Published, 10/01/2007.
  • Boudina S, Sena S, Theobald H, Sheng X, Wright JJ, Hu XX, Aziz S, Johnson JI, Bugger H, Zaha VG & Abel ED (2007). Mitochondrial energetics in the heart in obesity-related diabetes: direct evidence for increased uncoupled respiration and activation of uncoupling proteins. Diabetes. Vol. 56, 2457-66. Published, 09/01/2007.
  • Hao HX, Cardon CM, Swiatek W, Cooksey RC, Smith TL, Wilde J, Boudina S, Abel ED, McClain DA & Rutter J (2007). PAS kinase is required for normal cellular energy balance. Proceedings of the National Academy of Sciences of the United States of America. Vol. 104, 15466-71. Published, 09/01/2007.
  • Boudina S & Abel ED (2007). Diabetic cardiomyopathy revisited. Circulation. Vol. 115, 3213-23. Published, 06/01/2007.
  • Sano M, Izumi Y, Helenius K, Asakura M, Rossi DJ, Xie M, Taffet G, Hu L, Pautler RG, Wilson CR, Boudina S, Abel ED, Taegtmeyer H, Scaglia F, Graham BH, Kralli A, Shimizu N, Tanaka H, Mäkelä TP & Schneider MD (2007). Ménage-à-trois 1 is critical for the transcriptional function of PPARgamma coactivator 1. Cell metabolism. Vol. 5, 129-42. Published, 02/01/2007.
  • Lelliott CJ, Medina-Gomez G, Petrovic N, Kis A, Feldmann HM, Bjursell M, Parker N, Curtis K, Campbell M, Hu P, Zhang D, Litwin SE, Zaha VG, Fountain KT, Boudina S, Jimenez-Linan M, Blount M, Lopez M, Meirhaeghe A, Bohlooly-Y M, Storlien L, Strömstedt M, Snaith M, Oresic M, Abel ED, Cannon B & Vidal-Puig A (2006). Ablation of PGC-1beta results in defective mitochondrial activity, thermogenesis, hepatic function, and cardiac performance. PLoS biology. Vol. 4, e369. Published, 11/01/2006.
  • Boudina S & Abel ED (2006). Mitochondrial uncoupling: a key contributor to reduced cardiac efficiency in diabetes. Physiology (Bethesda, Md.). Vol. 21, 250-8. Published, 07/01/2006.
  • Boudina S, Sena S, O'Neill BT, Tathireddy P, Young ME & Abel ED (2005). Reduced mitochondrial oxidative capacity and increased mitochondrial uncoupling impair myocardial energetics in obesity. Circulation. Vol. 112, 2686-95. Published, 10/01/2005.
  • Leone TC, Lehman JJ, Finck BN, Schaeffer PJ, Wende AR, Boudina S, Courtois M, Wozniak DF, Sambandam N, Bernal-Mizrachi C, Chen Z, Holloszy JO, Medeiros DM, Schmidt RE, Saffitz JE, Abel ED, Semenkovich CF & Kelly DP (2005). PGC-1alpha deficiency causes multi-system energy metabolic derangements: muscle dysfunction, abnormal weight control and hepatic steatosis. PLoS biology. Vol. 3, e101. Published, 04/01/2005.
  • Mazumder PK, O'Neill BT, Roberts MW, Buchanan J, Yun UJ, Cooksey RC, Boudina S & Abel ED (2004). Impaired cardiac efficiency and increased fatty acid oxidation in insulin-resistant ob/ob mouse hearts. Diabetes. Vol. 53, 2366-74. Published, 08/01/2004.
  • Dos Santos P, Laclau MN, Boudina S & Garlid KD (2004). Alterations of the bioenergetics systems of the cell in acute and chronic myocardial ischemia. Molecular and cellular biochemistry. Vol. 256-257, 157-66. Published, 02/01/2004.
  • Dos Santos P, Kowaltowski AJ, Laclau MN, Seetharaman S, Paucek P, Boudina S, Thambo JB, Tariosse L & Garlid KD (2002). Mechanisms by which opening the mitochondrial ATP- sensitive K(+) channel protects the ischemic heart. American journal of physiology. Heart and circulatory physiology. Vol. 283, H284-95. Published, 06/01/2002.
  • Ducret T, Boudina S, Sorin B, Vacher AM, Gourdou I, Liguoro D, Guerin J, Bresson-Bepoldin L & Vacher P (2002). Effects of prolactin on intracellular calcium concentration and cell proliferation in human glioma cells. Glia. Vol. 38, 200-14. Published, 05/01/2002.
  • Boudina S, Laclau MN, Tariosse L, Daret D, Gouverneur G, Bonoron-Adèle S, Saks VA & Dos Santos P (2002). Alteration of mitochondrial function in a model of chronic ischemia in vivo in rat heart. American journal of physiology. Heart and circulatory physiology. Vol. 282, H821-31. Published, 02/01/2002.
  • Laclau MN, Boudina S, Thambo JB, Tariosse L, Gouverneur G, Bonoron-Adèle S, Saks VA, Garlid KD & Dos Santos P (2001). Cardioprotection by ischemic preconditioning preserves mitochondrial function and functional coupling between adenine nucleotide translocase and creatine kinase. Journal of molecular and cellular cardiology. Vol. 33, 947-56. Published, 05/01/2001.