DOUGLAS R MACKAY portrait
  • Research Associate Professor, Oncological Sciences
801-581-5441

Research Statement

I have been involved in research aimed at understanding the molecular regulation of abscission timing for 13 years, playing an integral role in key findings that demonstrate a link between nuclear pore reassembly and the abscission checkpoint. My research was also instrumental in uncovering a novel connection between postmitotic genome surveillance and the abscission checkpoint, which allowed me to characterize both Chk1 and ATR as factors upstream of Aurora B in abscission checkpoint signaling. Together, my contributions represent two previously uncharacterized physiological conditions where the abscission checkpoint is sustained, as well as the only known factors upstream of Aurora B in this context. I am currently collaborating with the Wes Sundquist Lab in the Department of Biochemistry to understand the function of novel proteins thought to be involved in the process of abscission and the abscission checkpoint. Connections between DNA damage signaling and abscission have fueled a parallel, yet synergistic interest in exploring how the nuclear pore components Nup153 and Nup50 are involved in the canonical DNA damage response.

Research Keywords

  • Cytokinesis
  • Abscission Checkpoint
  • Nuclear Pore Complex
  • DNA Damage
  • Cell Biology
  • Cell Cycle Regulation

Presentations

  • Mackay DR, Makise M, and Ullman KS. A checkpoint during cytokinesis monitors nuclear pore assembly. Dept. of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA. , , 2012.
  • Mackay DR, Makise M, and Ullman KS. A checkpoint during cytokinesis monitors nuclear pore assembly. Dept. of Biochemistry, Molecular, and Cellular Biology, University of Tennessee, Knoxville, TN, USA. , , 2012.
  • Mackay DR, Makise M, and Ullman KS. A checkpoint during cytokinesis monitors nuclear pore assembly. Dept. of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA. , , 2011.
  • Mackay DR and Ullman KS. Postmitotic genome surveillance regulates the abscission checkpoint through Chk1 activity. The 2014 ASCB/IFCB Annual Meeting, Philadelphia, PA. USA. , , 2014.
  • Mackay DR and Ullman KS. Replication stress triggers the abscission checkpoint. LDS Life Science Research Symposium, Salt Lake City, UT, USA. , , 2013.
  • Mackay DR, Howa AC, Werner TL, and Ullman KS. Nup153 and Nup50 promote recruitment of 53BP1 to DNA repair foci by antagonizing BRCA1-mediated events. Poster session presented at AACR DNA Repair: Tumor Development and Therapeutic Response Meeting, Montreal,Canada. Poster, Presented, 2016.
  • Mackay DR and Ullman KS. Replication stress leads to activation of the abscission checkpoint. Poster session presented at The American Society for Cell Biology Annual Meeting, New Orleans, LA, USA. Poster, Presented, 2013.
  • Mackay DR and Ullman KS. A role for the DNA damage response pathway in regulating the NPC-abscission checkpoint. Poster session presented at The Cell Cycle, Cold Spring Harbor, NY, USA. Poster, Presented, 2012.
  • Mackay DR, Makise M, and Ullman KS. Defects in late mitotic nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint. Poster session presented at The American Society for Cell Biology 50th Annual Meeting, Philadelphia, PA, USA. Poster, Presented, 2010.
  • Mackay DR, Elgort S, and Ullman KS. The nucleoporin Nup153 has separable roles in both early mitotic progression and the resolution of mitosis. Poster session presented at The American Society for Cell Biology 49th Annual Meeting, San Diego, CA, USA. Poster, Presented, 2009.
  • Mackay DR, Elgort S, and Ullman KS. The nuclear pore protein Nup153 has separable roles in both early mitotic progression and the resolution of mitosis. Poster session presented at The American Society for Cell Biology 48th Annual Meeting, San Francisco, CA, USA. Poster, Presented, 2008.
  • Mackay DR, Elgort S, and Ullman KS. Domain Analysis reveals two mitotic roles for the nucleoporin Nup153. Poster session presented at Molecular Cell Biology Gordon Research Conference, New London, NH, USA. Poster, Presented, 2008.
  • Mackay DR, Elgort S, and Ullman KS. Mitotic remodeling of the nuclear envelope. Poster session presented at The American Society for Cell Biology 47th Annual Meeting, Washington, DC, USA. Poster, Presented, 2007.
  • Mackay DR, Elgort S, Prunuske AJ, and Ullman KS. Mitotic remodeling of the nuclear envelope. Poster session presented at The American Society for Cell Biology 46th Annual Meeting, San Diego, CA, USA. Poster, Presented, 2006.
  • Mackay DR, Hu M, Li B, and Dai X. The mouse Ovol2 gene is required for neural development during embryogenesis. Poster session presented at Mouse Molecular Genetics Meeting, Cold Spring Harbor, NY, USA. Poster, Presented, 2004.
  • Mackay DR, Li B, and Dai X. A functional characterization of movo2. Poster session presented at Southwest Developmental Biology Meeting, Salt Lake City, UT, USA. Poster, Presented, 2003.
  • Mackay DR, Li B, and Dai X. A functional characterization of movo2. Poster session presented at Mouse Molecular Genetics Meeting, Cold Spring Harbor, NY, USA. Poster, Presented, 2002.

Languages

  • English, Fluent.

Publications

  • Mackay DR, Makise M, Ullman KS (date unknown). Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint. Vol. 191, 923-31. Accepted, .
  • Makise M, Mackay DR, Elgort S, Shankaran SS, Adam SA, Ullman KS (date unknown). The Nup153-Nup50 protein interface and its role in nuclear import. Vol. 287, 38515-22. Accepted, .
  • Mackay DR, Howa AC, Werner TL, Ullman KS (date unknown). Nup153 and Nup50 promote recruitment of 53BP1 to DNA repair foci by antagonizing BRCA1-dependent events. Vol. 130, 3347-3359. Accepted, .
  • Mackay DR, Ullman KS (date unknown). Coordinating postmitotic nuclear pore complex assembly with abscission timing. Vol. 2, 283-8. Accepted, .
  • Mackay DR, Ullman KS (date unknown). ATR and a Chk1-Aurora B pathway coordinate postmitotic genome surveillance with cytokinetic abscission. Vol. 26, 2217-26. Accepted, .
  • Mackay DR, Ullman KS, Rodesch CK (date unknown). Time-lapse imaging of mitosis after siRNA transfection.LID - 10.3791/1878 [doi]LID - 1878 [pii]. Accepted, .
  • Mackay DR, Elgort SW, Ullman KS (date unknown). The nucleoporin Nup153 has separable roles in both early mitotic progression and the resolution of mitosis. Vol. 20, 1652-60. Accepted, .
  • Shankaran SS, Mackay DR, Ullman KS (date unknown). A time-lapse imaging assay to study nuclear envelope breakdown. Vol. 931, 111-22. Accepted, .