VLADIMIR HLADY portrait
  • Professor, Biomedical Engineering
  • Adjunct Professor, Medicinal Chemistry

Publications

  • Rahman S, Fogelson A & Hlady V (2020). Effects of elapsed time on downstream platelet adhesion following transient exposure to elevated upstream shear forces. Colloids and surfaces. B, Biointerfaces. Vol. 193, 111118. Published, 07/01/2020.
    https://doi.org/10.1016/j.colsurfb.2020.111118
  • S.M. Rahman & V. Hlady (2019). Downstream platelet adhesion and activation under highly elevated upstream shear forces. Acta Biomaterialia, Elsevier. Vol. 91, 135-143. Published, 04/17/2019.
    https://doi.org/10.1016/j.actbio.2019.04.028
  • Sakia, Jiban, Mohammadpour, Raziye, Yazdimamaghani, Mostafa, Northrup, Hannah, Hlady, Vladimir & Ghandehari, Hamidreza (2018). Silica Nanoparticle–Endothelial Interaction: Uptake and Effect on Platelet Adhesion under Flow Conditions. ACS. Vol. 1(5), 1620–1627. Published, 10/30/2018.
    https://pubs.acs.org/doi/abs/10.1021/acsabm.8b0046...
  • Rahman SM, Eichinger CD & Hlady V (2018). Effects of upstream shear forces on priming of platelets for downstream adhesion and activation. Acta biomaterialia. Vol. 73, 228-235. Published, 04/11/2018.
  • Hsiao TW & Tresco PA (2017). Astrocyte spreading and migration on aggrecan-laminin dot gradients. AVS. Vol. 13(1). Published, 07/17/2017.
    http://dx.doi.org/10.1116/1.5001675
  • Eichinger CD & Hlady V (2017). Binary agonist surface patterns prime platelets for downstream adhesion in flowing whole blood. AVS. Vol. 12. Published, 04/28/2017.
    http://dx.doi.org/10.1116/1.4982596
  • Kathleen M. Job, Ryan O’Callaghan, Vladimir Hlady, Alexandra Barabanova, and Randall O. Dull, The Biomechanical Effects of Resuscitation Colloids on the Compromised Lung Endothelial Glycocalyx, Anesth Analg. 2016:123:382-93. Published, 08/2016.
  • Colin Eichinger, Aaron Fogelson and Vladimir Hlady, Functional assay of antiplatelet drugs based on margination of platelets in flowing blood, Biointerphases, 11, 029805 (2016); doi: 10.1116/1.4945305. Published, 03/30/2016.
    http://dx.doi.org/10.1116/1.4945305
  • Gooch NW, Hlady V, "Two surface gradients of polyethylene glycol for a reduction in protein adsorption," Surface Innovations 2015;3(SI3):172-180;. Published, 08/15/2015.
  • Hsiao TW, Swarup VP, Eichinger CD, Hlady V, Cell substrate patterning with glycosaminoglycans to study their biological roles in the central nervous system. Methods Mol Biol 2015;1229:457-67. Published, 05/01/2015.
  • T.W. Hsiao, P.A. Tresco, and V. Hlady, Astrocytes alignment and reactivity on collagen hydrogels patterned with ECM proteins, Biomaterials 2015;39, 124-130. Published, 01/01/2015.
  • V.P. Swarup, T.W. Hsiao, J.-X. Zhang, G.D. Prestwich, B. Kuberan, and V. Hlady, Exploiting Differential Surface Display of Chondroitin Sulfate Variants for Directing Neuronal Outgrowth, J. Am. Chem. Soc. 2013; 135(36); 13488–13494. Published, 09/15/2014.
  • T.W. Hsiao, V.P. Swarup, B. Kuberan, P.A. Tresco, and V. Hlady, Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans. Acta Biomater 2013;9(7):7200-8. Published, 09/15/2013.
  • Swarup VP, Hsiao TW, Zhang J, Prestwich GD, Kuberan B, Hlady V, Exploiting differential surface display of chondroitin sulfate variants for directing neuronal outgrowth. J Am Chem Soc 2013 Sep 11;135(36):13488-94. Published, 09/2013.
    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...
  • X.-Z. Du, D.W. Britt, V. Hlady, Annexin A5 Binding and Rebinding to Mixed Phospholipid Monolayers Studied by SPR and AFM, peer reviewed chapter in: Proteins at Interfaces III State of the Art, (Eds: T. Horbett, J.L. Brash, W.Norde), ACS Symposium Series, 2013; Vol. 1120, Ch. 19, pp 419–432. Published, 08/2013.
  • Hsiao TW, Swarup VP, Kuberan B, Tresco PA, Hlady V, Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans. Acta Biomater 2013 Jul;9(7):7200-8. Published, 07/2013.
    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...
  • Job KM, Dull RO, Hlady V, Use of reflectance interference contrast microscopy to characterize the endothelial glycocalyx stiffness. Am J Physiol Lung Cell Mol Physiol 2012 Jun 15;302(12):L1242-9. Published, 07/2012.
    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...
  • C.D. Eichinger, T.W. Hsiao, V. Hlady, Multiprotein microcontact printing with micrometer resolution, Langmuir, 2012;28(4):2238-43. Published, 12/28/2011.
    http://www.ncbi.nlm.nih.gov/pubmed/22204564
  • L.E. Corum, V. Hlady, The effect of upstream platelet-­fibrinogen interactions on downstream adhesion and activation, Biomaterials, 2012;33:1255-1260. Published, 12/2011.
    http://www.ncbi.nlm.nih.gov/pubmed/22100981
  • F-W. Meng, V. Hlady, P.A. Tresco, Surface Ligands Patterned on Biomaterials Induce Alignment in Astrocyte Tissue Constructs, Biomaterials, 2012;33:1323-1335. Published, 11/2011.
    http://www.ncbi.nlm.nih.gov/pubmed/22100982
  • Y-X. Ding, V. Hlady. Competitive adsorption of three human plasma proteins onto sulfhydryl-to-sulfonate gradient surfaces, Croat Chem Acta, 2011;84(2):193-202. Published, 11/2011.
    http://www.ncbi.nlm.nih.gov/pubmed/22279244
  • R. O’Callaghan, K. M. Job, R. O. Dull, V. Hlady. Stiffness and heterogeneity of the pulmonary endothelial glycocalyx measured by atomic force microscopy. AJP Lung Cell Mol Physiol, 301 (2011) L353–L360. Published, 06/2011.
    http://www.ncbi.nlm.nih.gov/pubmed/21705487
  • L.E. Corum, C.D. Eichinger, T.W. Hsiao, V. Hlady, Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation, Langmuir 27 (13) (2011) 8316–8322. Published, 06/2011.
    http://www.ncbi.nlm.nih.gov/pubmed/21657213
  • R.J. Stewart, T.C. Ransom, V. Hlady, Natural Underwater Adhesives (review), J Polym Sci Part B: Polym Phys 49: 757–771, 2011. Published, 06/2011.
    http://www.ncbi.nlm.nih.gov/pubmed/21643511
  • C. Miller, Lj. Komunjer, V. Hlady, "Heterogeneous nucleation of calcium phosphate dihydrate on modified silica surfaces" Med Vjesn 2010; 42(3-4):63-72. Published, 08/2010.
  • V. Deshmukh, D. W. Britt, V. Hlady, "Excess fibrinogen adsorption to monolayers of mixed lipids" Colloids and Surfaces B: Biointerfaces 81 (2010) 607–613. Published, 08/2010.
    http://www.ncbi.nlm.nih.gov/pubmed/20829000
  • Y-X. Ding, S. Streitmatter, B.E. Wright, V. Hlady, "Spatial Variation of the Charge and Sulfur Oxidation State in 2 a Surface Gradient Affects Plasma Protein Adsorption" Langmuir 2010, 26(14), 12140–12146. Published, 06/2010.
    http://www.ncbi.nlm.nih.gov/pubmed/20568822
  • L.E. Corum, V. Hlady, Screening platelet - surface interactions using negative surface charge gradients. Biomaterials 31 (2010) 3148–3155. Published, 02/2010.
    http://www.ncbi.nlm.nih.gov/pubmed/20149436
  • U. Maran, D.W. Britt, C.B. Fox, J.M. Harris, A.M. Orendt, H. Conley, R. Davis, V. Hlady, and P.J. Stang, Self-assembly of a triangle-shaped, hexaplatinum-incorporated, supramolecular amphiphile in solution and at interfaces. Chemistry 15(34) (2009) 8566-77. Published, 08/2009.
    http://www.ncbi.nlm.nih.gov/pubmed/19655346
  • G.N. Hodgkinson, V. Hlady, Relating protein adsorption to material's surface heterogeneity. I. The effect of annealing of micro-contact printed OTS pattern, J. Adhesion Science and Technology, 19(3-5) 2005, 235-255. Published, 08/2008.
    http://www.ncbi.nlm.nih.gov/pubmed/19693285
  • G.N. Hodgkinson, P.A. Tresco, V. Hlady, The influence of sub-micron inhibitory clusters on growth cone substratum attachments and CD44 expression. Biomaterials 29(31) (2008) 4227-35. Published, 08/2008.
    http://www.ncbi.nlm.nih.gov/pubmed/18694596
  • G. Huang, B.J. Endrizzi, V. Hlady, and R.J. Stewart, Formation of Biofunctional Thin Films on Gold Electrodes by Electrodeposition of Poly(acrylamide-co-tyrosineamide). Macromolecules 41 (2008) 448 - 452. Published, 06/2008.
    http://pubs.acs.org/doi/abs/10.1021/ma702205p
  • G. Hodgkinson, V. Hlady. The effects of proteoglycan surface patterning on neuronal pathfinding. (peer reviewed), Materialenwissenschaft und Werkstofftechnik, 38(12) (2007) 975 - 982. Published, 12/2007.
    http://www.ncbi.nlm.nih.gov/pubmed/20119506
  • G.N. Hodgkinson, V. Hlady, How surface heterogeneity affects protein adsorption: annealing of OTS patterns and albumin adsorption kinetics. Croatica Chemica Acta, 80(3-4) (2007) 405-20. Published, 11/2007.
    http://www.ncbi.nlm.nih.gov/pubmed/19746205
  • M. Stevens, R. Steren, V. Hlady, R. Stewart: Multiscale structure of the underwater adhesive of Phragmatopoma Californica: a nanostructured latex with a steep microporosity gradient, Langmuir. 23(9) (2007) 5045-9. Published, 09/2007.
    http://pubs.acs.org/cgi-bin/abstract.cgi/langd5/20...
  • N.W. Turner, X. Liu, S.A. Piletsky, V. Hlady, and D.W. Britt, Recognition of conformational changes in beta-lactoglobulin by molecularly imprinted thin films. Biomacromolecules, 8(9) (2007) 2781-7. Published, 09/2007.
    http://pubs.acs.org/cgi-bin/abstract.cgi/bomaf6/20...
  • A.P. Stevens, V. Hlady, R.O. Dull, Fluorescence correlation spectroscopy can probe albumin dynamics inside lung endothelial glycocalyx. Am J Physiol Lung Cell Mol Physiol 293 (2007) L328-35. Published, 08/2007.
    http://www.ncbi.nlm.nih.gov/pubmed/17483194
  • N.W. Turner, B.E. Wright, V. Hlady, and D.W. Britt: Formation of protein molecular imprints within Langmuir monolayers: A quartz crystal microbalance study, J. Colloid Interface Sci., 308 (2007) 71–80. Published, 04/2007.
    http://www.ncbi.nlm.nih.gov/pubmed/17204279
  • G.N. Hodgkinson, P.A. Tresco and V. Hlady, The differential influence of colocalized and segregated dual protein signals on neurite outgrowth on surfaces, Biomaterials 28 (2007) 2590–2602. Published, 03/2007.
    http://www.ncbi.nlm.nih.gov/pubmed/17316787
  • V. Hlady, G. Jogikalmath. Albumin binding and insertion into PS-b-PEO monolayers at air-water interface, Colloids and Surfaces B. Biointeractions, 54 (2007) 179–187. Published, 02/2007.
    http://www.ncbi.nlm.nih.gov/pubmed/17141488
  • N.W. Turner, C.W. Jeans, K.R. Brain, C.J. Allender, V. Hlady, and D.W. Britt: From 3D to 2D: A Review of the Molecular Imprinting of Proteins, Biotechnol. Prog 22(6) (2006) 1474-1489. Published, 11/2006.
    http://www.ncbi.nlm.nih.gov/pubmed/17137293

Research Statement

Research in Hlady's lab focuses on proteins and other macromolecules at biomaterial-host interfaces. Recent research themes include: - effects of biomaterials surface heterogeneity on the proteins-surface interactions and dynamics, - characterization of materials interfaces using adhesion and elasticity mapping at nanometer resolution, - controlling blood proteins interactions with surfaces by using

Presentations

  • Shekh Rahman and Vladimir Hlady "Effects of Highly Elevated Upstream Shear Forces on Priming of Platelets for Downstream Adhesion and Activation" UBEC symposium, Salt Lake City, UT. Other, Presented, 12/07/2018.
  • Alexandra Zudova, Elizabeth Pumford, and Vladimir Hlady "Upstream Platelet Priming Effects on Transient Downstream Platelet-Surface Interactions" UBEC symposium, Salt Lake City, UT. Other, Presented, 12/07/2018.
  • Elizabeth Pumford, Alexandra Zudova, and Vladimir Hlady "Combined effects of transient upstream priming and hematocrit on downstream platelet rolling" UBEC Symposium, Salt Lake City, UT. Other, Presented, 12/07/2018.
  • Hannah Northrup, Susan Bock & Vladimir Hlady "End-Point Coupling of Heparin to the Surface of a Microfluidics Device for Studying Platelet Priming" UBEC symposium, Salt Lake City, UT. Other, Presented, 12/07/2018.
  • Youseph Yazdi, Tejal Desai, Vladimir Hlady, George Truskey, Tom Skalak, Frank Yin, Daniel Kramer, G Burtt Holmes, Peter G Katona "The Whitaker International Program: Outcomes and Lessons from 10 Years of Support for International Biomedical Research" BMES 2018 meeting, Atlanta, GE. Other, Presented, 10/19/2018.
  • Alexandra Zudova, Elizabeth Pumford, and Vladimir Hlady "Upstream Platelet Priming Effects on Transient Downstream Platelet-Surface Interactions" BMES 2018 meeting, Atlanta, GE. Other, Presented, 10/17/2018.
  • Shekh Rahman and Vladimir Hlady "Effects of Highly Elevated Upstream Shear Forces on Priming of Platelets for Downstream Adhesion and Activation" BMES 2018 meeting, Atlanta, GE. Other, Presented, 10/17/2018.
  • Shekh Rahman, Colin Eichinger, Vladimir Hlady "Evaluation of Upstream Stenosis-Platelet Interactions for Downstream Adhesion and Activation" BMES 2017 meeting, Phoenix, AR. Other, Presented, 10/16/2017.
  • Alexandra Zudova, Elizabeth Pumford, Vladimir Hlady 'Upstream Platelet Priming Effects on Transient Downstream Platelet-Surface Interactions' BMES 2017 meeting, Phoenix, AR. Other, Presented, 10/16/2017.
  • S. Rahman, C. Eichnger and V. Hlady. Effects of Upstream Shear Forces in Priming Platelets for Downstream Activation and Adhesion. UBEC, November 2016 Salt Lake City, UT. Other, Presented, 12/2016.
  • R. Goldstein, V. Hlady and T. Deans. Engineering a bioinspired bone marrow microenvironent for enhanced stem cell differentiation. 2016 Annual Meeting Biomedical Engineering Society, October 5-8, 2016, Minneapolis, Minn. Other, Presented, 10/06/2016.
  • "Surface modifications for different applications in bioengineering” TIMR Department, Universite de Technologie de Compiegne, Compiegne, France. Invited Talk/Keynote, Presented, 06/05/2015.
  • “Understanding axonal pathfinding for improving neuronal regeneration” BioMécanique et BioIngénierie (BMBI) Department, Universite de Technologie de Compiegne, Compiegne, France. Invited Talk/Keynote, Presented, 05/13/2015.
  • “Engineering interfaces for biomedical applications” Laboratoire de Réactivité de Surface, Universite Pierre et Marie Currie, Paris, France. Invited Talk/Keynote, Presented, 05/11/2015.
  • “Engineering of Biointerfaces” IMID group, Universite de Technologie de Compiegne, Compiegne, France. Invited Talk/Keynote, Presented, 05/07/2015.
  • V.P. Swarup, V.M. Tran, M. Kalita, M.V. Quintero, V. Hlady and K. Balagurunathan. "Modulating CSPG biosynthesis in reactive astrocytes to enhance neuronal regeneration." Biomedical Engineering Society Annual Meeting, September 28 – October 1, 2013, Seattle, WA. Other, Presented, 09/2013.
  • V.P. Swarup, T.W. Hsiao, J-X. Zhang, G. Prestwich, K. Balagurunathan and V. Hlady. "Exploiting sulfation pattern of chondroitin sulfate proteoglycans for vectorial growth of neurons." Gordon Research Conference on Biomaterials & Tissue Engineering, July 28 – August 2, 2013, Holderness, New Hampshire. Other, Presented, 07/2013.
  • C. Eichinger, T.W. Hsiao, V. Hlady, "Multi-protein microcontact printing of surfaces for neuronal regeneration studies," poster presented at Utah Biomedical Engineering Conference, Salt Lake City, 09/10/2011. Other, Presented, 09/10/2011.
  • K.M. Job, B. Bach, R. O’Callaghan, R.O. Dull, V. Hlady, "Characterization of the Lung Microvascular Endothelial Glycocalyx: Studies with Albumin and HES," poster presented at Utah Biomedical Engineering Conference, Salt Lake City, 09/10/2011. Other, Presented, 09/10/2011.
  • V. Swarup, T.W. Hsiao, J-X. Zhang, G. Prestwich, K. Balagurunathan, V. Hlady, "Differential action of CS A, CS C and CS E on neuronal outgrowth and pathfinding," poster presented at Utah Biomedical Engineering Conference, Salt Lake City, 09/10/2011. Other, Presented, 09/10/2011.
  • T.W. Hsiao, V. Hlady, "Astrocyte Expression of Chondroitin Sulfate Proteoglycan in Response to Surface Adsorbed Fibrinogen in vitro," podium presentation at Utah Biomedical Engineering Conference, Salt Lake City, 09/10/2011 . Other, Presented, 09/10/2011.
  • "Engineering interfaces for biomedical applications" presented at International Scientific Symposium "From Ruđer Bošković to Today: Contribution of Croatian Scientists to the World Scientific Heritage," Dubrovnik, May 29  -­‐  June 2, 2011 . Invited Talk/Keynote, Presented, 06/01/2011.
    http://rb300.irb.hr/speakers.html
  • Vladimir Hlady ""Engineering of bio-interfaces for neuronal pathfinding and hemocompatibility" undergraduate seminar, Dept of Bioengineering. Other, Presented, 10/2010.
  • Tony Hsiao, Patrick Tresco, and Vladimir Hlady "Neuronal Pathfinding on Dot Gradients of Mixed Permissive and Inhibitory Protein Cues" poster at Mountain West BME Conference, Canyons, Utah, 2010. Other, Presented, 09/2010.
  • Lindsey Corum, Krystin Meidell, Ashley Cook, Vladimir Hlady, "Upstream platelet surface contacts prime platelets for downstream adhesion in vitro" poster at Mountain West BME Conference, Canyons, Utah, 2010 . Other, Presented, 09/2010.
  • Vimal Swarup, Tony Hsiao, Kuberan Balagurunathan Vladimir Hlady, "Stamped proteoglycans as tools for neuronal outgrowth studies" poster at Mountain West BME Conference, Canyons, Utah, 2010 . Other, Presented, 09/2010.
  • Colin Eichinger, Nathan Gooch, Vladimir Hlady, "Adhesion mapping and XPS analysis of grafted PEO" poster at Mountain West BME Conference, Canyons, Utah, 2010. Other, Presented, 09/2010.
  • Kathleen 
M.
 Job,
 Ryan 
O’Callaghan, 
Randal
 Dull,
 Vladimir 
Hlady, "Biomechanical 
Effects 
of 
Albumin 
to 
the 
Lung
 Microvascular
 Endothelial
 Glycocalyx
"
 poster at Mountain West BME Conference, Canyons, Utah, 2010. Other, Presented, 09/2010.
  • “Screening Protein Surface Interactions using Surface Chemistry Gradients”, invited talk, Gordon Research Conference "Biomaterials: Biocompatibility / Tissue Engineering," Holderness School, NH. Invited Talk/Keynote, Presented, 07/19/2009.

Geographical Regions of Interest

  • Croatia
  • France